|
KMID : 0613820030130050699
|
|
Journal of Life Science
2003 Volume.13 No. 5 p.699 ~ p.704
|
|
|
Enhanced Discrimination of Listeria spp. Using RAPD Fingerprinting Complemented by Ribotyping-PCR
|
|
Lim Hyung-Kun
Hong Chong-Hae
Bahk Gyung-Jin
Choi Won-Sang
|
|
|
Abstract
|
|
|
|
The results typed by random amplification of polymorphic DNA (RAPD) were compared with those obtained by Enterobacterial repititive intergenic consensus (ERIC) fingerprinting and ribotyping-PCR. The discriminatory power of RAPD typing was the best among the methods tested. RAPD typing with two different primers for 13 Listeria spp. reference strains produced 11 patterns each. In contrast, ERIC fingerprinting produced 9 patterns and ribotyping-PCR produced 7 patterns each. Composite of two separate RAPD (Lis 11 and primer 6) results or RAPD (Lis11)/ ribotyping-PCR differentiated all 13 Listeria spp. reference strains. Therefore, composite of 2 separate RAPD (Lis11 and primer 6) or composite of RAPD (Lis11)/ribotyping-PCR is expected the most promising approach for typing field isolated Listeria spp. strains.
|
|
|
KEYWORD
|
|
|
random amplification of polymorphic DNA (RAPD), Enterobacterial repetitive intergenic consensus, (ERIC) fingerprinting, ribotyping-PC
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|